Anti-Human Adiponectin

Source: Polyclonal Rabbit 

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Adiponectin. Anti-Human Adiponectin-specific antibody was purified by affinity chromatography employing an immobilized Human Adiponectin matrix. 

Immunogen: Hi-5 Insect cells derived Recombinant Human Adiponectin 

Sandwich ELISA: To detect Human Adiponectin by sandwich ELISA (using 100μl/well) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Adiponectin (500-P239BT) as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human Adiponectin. 

Western Blot: To detect Human Adiponectin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Adiponectin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 

Note: 

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.