Biotinylated Anti-Human Amphiregulin

Source: Polyclonal Rabbit 

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Amphiregulin. Anti-Human Amphiregulin-specific antibody was purified by affinity chromatography and then biotinylated. 

Immunogen: E.coli derived Recombinant Human Amphiregulin

Sandwich ELISA: To detect Human Amphiregulin by sandwich ELISA (using 100μl/well), a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human Amphiregulin (500-P322) as a capture antibody, allows the detection of at least 2000-4000 pg/ml ofRecombinant Human Amphiregulin. 

Western Blot: To detect Human Amphiregulin by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human Amphiregulin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.