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Anti-Human IFN-γ

Anti-Human IFN-γ

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Anti-Human IFN-γ

产品详情

产品编号:QC500-P32

Source: Polyclonal Rabbit 

 

 

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Human IFN-γ. Anti-Human IFN-γ specific antibody was purified by affinity chromatography employing immobilized Human IFN-γ matrix. 

 

 

Immunogen: E.coli derived Recombinant Human IFN-γ 

 

 

Sandwich ELISA: To detect Human IFN-γ by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IFN-γ (500-P32Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-γ. 

 

 

Western Blot: To detect Human IFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-γ is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 

 

 

Note: 

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Cross Reactivity:
Bacteria, Human
Research Interest