抗体
Biotinylated Anti-Rat IL-2
Biotinylated Anti-Rat IL-2
产品详情
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat IL-2. Anti-Rat IL-2 specific antibody was purified by affinity chromatography and then biotinylated.
Source: Polyclonal Rabbit
Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat IL-2. Anti-Rat IL-2 specific antibody was purified by affinity chromatography and then biotinylated.
Immunogen: E.coli derived Recombinant Rat IL-2
Sandwich ELISA: To detect Rat IL-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Rat IL-2 (500-P274) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IL-2.
Western Blot: To detect Rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.