Anti-Human IL-12

Source: Polyclonal Goat 

Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL12. Anti-Human IL-12 specific antibody was purified by affinity chromatography employing immobilized hIL12 matrix. 

Immunogen: CHO cells derived Recombinant Human IL-12 p70 

Sandwich ELISA: To detect hIL-12 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-12 (500-P154GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12. 

Western Blot: To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 

Note: 

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.