Biotinylated Anti-Human IL-17D

Source: Polyclonal Rabbit 

Preparation: Produced from sera of rabbits immunized with highly pure recombinant Human IL-17D. Anti-Human IL-17D specific antibody was purified by affinity chromatography and then biotinylated. 

Immunogen: E.coli derived Recombinant Human IL-17D 

Sandwich ELISA: To detect Human IL-17D by sandwich ELISA (using 100 ml/well) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti- Human IL-17D (500-P88) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human IL-17D. 

Western Blot: To detect Human IL-17D by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. when used in conjunction with compatible development reagents the detection limit for recombinant Human IL-17D is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.