Anti-Human Wnt-3a

Source: Polyclonal Rabbit 

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWnt-3a. Anti-Human Wnt-3a specific antibody was purified by affinity chromatography employing immobilized hWnt-3a matrix. 

Immunogen: E.coli derived Recombinant Human Wnt-3a 

Sandwich ELISA: To detect hWnt-3a by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Wnt-3a (500-P251Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWnt-3a. 

Western Blot: To detect hWnt-3a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWnt-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. 

Note: 

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.