Recombinant Mouse Gasdermin D (GSDMD)

Recombinant Gasdermin D (GSDMD)

Organism Species: Mus musculus (Mouse)

Instruction manual

FOR RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Met1~Gln325

Tags: N-terminal His Tag

Subcellular Location: Membrane, Cytoplasm, Extracellular matrix

Purity: > 95%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

Original Concentration: 120µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.0

Predicted Molecular Mass: 39.5KDa

Accurate Molecular Mass: 40kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not

vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined

by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no

obvious degradation and precipitation were observed. The loss rate is less than 5% within the

expiration date under appropriate storage condition.