| 质粒类型: | 酵母双杂交系统 |
|---|---|
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 7.9kb |
| 5' 测序引物及序列: | T7 Forward |
| 载体标签: | c-Myc |
| 载体抗性: | Kanamycin (卡纳霉素) |
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| QC1643 | pGBKT7-Lam |
5ug质粒 |
¥1000.00 |
pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.