The pIRES-hrGFP vectors contain a dicistronic expression cassette in which the multiple cloning site (MCS) is followed by the EMCV-IRES linked to the hrGFP coding sequence. This design allows the expression of a gene of interest to be monitored at the single-cell level due to expression of hrGFP on the same transcript. The gene of interest may be fused to three contiguous copies of either the FLAG® epitope (pIRES-hrGFP-1a) or the HA epitope (pIRES-hrGFP-2a).