| 质粒类型: | 慢病毒载体 |
|---|---|
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | TET |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8226 bp |
| 5' 测序引物及序列: | EGFP-C Forward: CATGGTCCTGCTGGAGTTCGTG |
| 载体标签: | GFP |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| QC1454 | pPRIME-TET-GFP-FF3 |
5ug质粒 |
¥1000.00 |
TET promoter (minimal CMV promoter containing seven upstream Tet-operator sites) transcribes GFP and miR30-based shRNA targeting FF3. The FF3 hairpin can be replaced with any other hairpin by cloning into the EcoR1/Xho1 site.
Please note that the full plasmid sequence shown here is for the empty vector only and does not contain the FF3 targeting sequence (AGCTCCCGTGAATTGGAATCCTAGTGAAGCCACAGATGTAGGATTCCAATTCAGCGGGAGCC)