| 质粒类型: | 大肠杆菌表达载体 |
|---|---|
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5260 bp |
| 5' 测序引物及序列: | T7: 5'-TAATACGACTCACTATAGGG-3' |
| 3' 测序引物及序列: | T7t: 5'-TGCTAGTTATTGCTCAGCGG-3' |
| 载体标签: | N-His,N-EK, C-S |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 备注: |
N-term HisTag with minimal extraneous sequences.
|
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| QC1939 | pET-45b(+) |
5ug质粒 |
¥1000.00 |
The pET-45b(+) vector carries an N-terminal His•Tag® coding sequence followed by an enterokinase cleavage site and an optional C-terminal S•TagTM sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 Terminator Primer (Cat. No. 69337-3).