质粒载体

pET-33b(+) 载体

pET-33b(+) 载体

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质粒类型: 大肠杆菌表达载体
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5383 bp
5' 测序引物: T7
5' 测序引物序列: T7: 5'-TAATACGACTCACTATAGGG-3'
载体标签: His (中间和C端), N-Thrombin
载体抗性: Kanamycin (卡那霉素)
备注:
Production of target proteins suitable for site-specific 32P-labeling; Nterm thrombin cleavage site; Nterm enterokinase cleavage site
产品编号  产品名称  规格  价格 
QC1948  pET-33b(+) 

5ug质粒

¥1000.00 
pET-33b(+) 质粒图谱
pET-33b(+) 载体图谱

The pET-33b(+) vector (Cat. No. 69054-3) is derived from pET-28b(+) and carries a 15bp sequence encoding the protein kinase A (PKA) site RRASV, located between the thrombin cleavage and Nde I sites (1). Proteins expressed in pET-33b(+) can be easily purified by metal chelation chromatography (via either N- or C-terminal His•Tag® sequences) and efficiently labeled with 32P- or 33P-gATP and the catalytic subunit of cAMP-dependent protein kinase from heart muscle. Labeled proteins can be used as direct probes in protein-protein interaction studies. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/ expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).