质粒载体

pTet-On-Advanced 载体

pTet-On-Advanced 载体

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质粒类型: 四环素调控系统
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 7139 bp 
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Neomycin(新霉素)
产品编号  产品名称  规格  价格 
QC1603  pTet-On-Advanced 

5ug质粒

¥1000.00 
pTet-On-Advanced质粒图谱

pTet-On-Advanced expresses an improved version of the reverse Tet (tetracycline)-controlled transactivator protein (rtTA), called rtTA-Advanced (1–4). It is more sensitive to doxycycline (Dox) and yields lower background expression than the original rtTA used in the Tet-On® System (2). The rtTA-Advanced protein is a fusion of amino acids 1–207 of a mutant Tet repressor (TetR) and 39 amino acids containing three minimal "F"-type transcriptional activation domains from the VP16 protein of herpes simplex virus. It is fully synthetic, lacks cryptic splice sites, and is codon-optimized for stable expression in mammalian cells.

载体应用

The pTet-On-Advanced Vector is used to develop stable Tet-On Advanced cell lines, which are hosts for a Dox-induced gene expression system. Once a vector containing a gene of interest under control of a Tet-responsive element (e.g., TRE-Tight or TRE2) is transfected into a Tet-On Advanced cell line, rtTA-Advanced binds to the TRE, and activates transcription of the gene of interest in the presence of Dox in a highly dose-dependent manner. Additional information on TRE-containing vectors and protocols describing the construction of Tet-On Advanced cell lines can be found in the Tet-On Advanced Inducible Gene Expression System User Manual (PT3898-1).