| 质粒类型: | 果蝇细胞表达系统 |
|---|---|
| 启动子: | P-AC5 (Actin 5C) |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5379bp |
| 5' 测序引物及序列: | AC5: 5'd[ACACAAAGCCGCTCCATCAG]3' |
| 载体标签: | V5 (Cterm), His (Cterm) |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| QC1088 | pAc5.1/V5-His B |
5ug质粒 |
¥1000.00 |
The DES®-Constitutive Kit provides the vector pAc5.1/V5-His for high-level constitutive expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. The vector offers the strong, constitutive promoter from the Drosophila actin 5C gene. The pAc5.1/V5-His vector also offers the following features:
- Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency
- C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
- C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.