质粒载体

pACYCDuet-1 载体

pACYCDuet-1 载体

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质粒类型: 大肠杆菌表达载体(双启动子)
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4008 bp 
5' 测序引物及序列: T7: 5'-TAATACGACTCACTATAGGG-3';
3' 测序引物及序列: T7t: 5'-TGCTAGTTATTGCTCAGCGG-3'
载体标签: N-His, C-S
载体抗性: 氯霉素(Chloramphenicol)
备注:
pACYCDuet-1 is designed for the coexpression of two target genes. pACYCDuet-1 carries the P15A replicon, lacI gene and chloramphenicol resistance gene.
产品编号  产品名称  规格  价格 
QC1236  pACYCDuet-1 

5ug质粒

¥1000.00 
pACYCDuet-1 质粒图谱
pACYCDuet-1 Vector Map

pACYCDuet™-1 is designed for the coexpression of two target genes. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7 promoter/lac operator and ribosome binding site (rbs). The vector also carries the P15A replicon, lacI gene and chloramphenicol resistance gene. This vector can be used in combination with pETDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of up to 4 target genes. Genes inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179- 3). Genes inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3)