质粒类型: | 哺乳细胞表达载体 |
---|---|
启动子: | CMV |
表达水平: | 高 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 5514 bp |
5' 测序引物: | pcDNA3.1-F:CTAGAGAACCCACTGCTTAC |
3' 测序引物: | pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG |
载体标签: | N-His, N-EK |
载体抗性: | Ampicillin (氨苄青霉素) |
筛选标记: | Neomycin (G418) |
质粒载体
pcDNA3.1/His A 载体
产品编号 | 产品名称 | 规格 | 价格 |
---|---|---|---|
QC1974 | pcDNA3.1/His A |
5ug质粒 |
¥1000.00 |
pcDNA3.1/His A, B, and C are 5.5 kb vectors derived from pcDNA3.1 and designed for high-level expression and purification of recombinant proteins in mammalian hosts. The vectors are supplied in three reading frames to facilitate in frame cloning with a polyhistidine metal-binding tag. The human cytomegalovirus (CMV) immediate-early promoter provides high-level expression in a wide range of mammalian cells. In addition, the vector replicates episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/His lacZ, is the pcDNA3.1/His B vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the N-terminal peptide. pcDNA3.1/His lacZ is included for use as a positive control for transfection, expression, and purification in the cell line of choice.