| 质粒类型: | 哺乳细胞表达载体 |
|---|---|
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5597 bp |
| 5' 测序引物: | T7 Fwd:5'-TAATACGACTCACTATAGGG-3' |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 筛选标记: | Hygromycin (潮霉素) |
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| QC1979 | pcDNA3.1/Hygro(+) |
5ug质粒 |
¥1000.00 |
This pcDNA3.1/Hygro(+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Hygromycin selectable marker and a reverse-orientation multiple cloning site.
The pcDNA™3.1 Expression Vector Family
Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:
- Cytomegalovirus (CMV) enhancer-promoter for high-level expression
- Large multiple cloning site in either forward (+) or reverse (-) orientations
- Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
- SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e., COS-1 and COS-7)
- Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli