| 质粒类型: | 哺乳动物表达载体 |
|---|---|
| 启动子: | CMV |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5015 bp |
| 5' 测序引物: | pcDNA3.1-F:CTAGAGAACCCACTGCTTAC |
| 3' 测序引物: | pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 筛选标记: | Zeocin 博来霉素 |
| 备注: |
Differs from other pcDNA3.1 in drug resistance; +/- refers to orientation of f1 ori.
|
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| QC1012 | pcDNA3.1/Zeo(+) |
5ug质粒 |
¥1000.00 |
This pcDNA™3.1/Zeo(+)vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Zeocin™ selectable marker and a forward-orientation multiple cloning site.
The pcDNA™3.1 Expression Vector Family
Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:
- Cytomegalovirus (CMV) enhancer-promoter for high-level expression
- Large multiple cloning site in either forward (+) or reverse (-) orientations
- Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
- SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e.,COS-1 and COS-7)
- Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli