别名: | pEF1/myc-HisB |
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质粒类型: | 哺乳动物表达载体 |
表达水平: | 高拷贝 |
启动子: | EF-1a |
载体大小: | 6169 bp |
5' 测序引物及序列: | T7 Fwd:5'd[TAATACGACTCACTATAGGG]3' |
载体标签: | 6X His, myc |
载体抗性: | Ampicillin (氨苄青霉素) |
筛选标记: | Neomycin |
产品编号 | 产品名称 | 规格 | 价格 |
---|---|---|---|
QC1037 | pEF1/myc-His B |
5ug质粒 |
¥1000.00 |
pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
- Human elongation factor 1?-subunit (hEF-1α) promoter for high-level expression across a broad range of species and cell types (Goldman et al., 1996; Mizushima and Nagata, 1990).
- Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc (c-myc) epitope and a polyhistidine metal-binding peptide.
- Neomycin resistance gene for selection of stable cell lines.
- Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g., COS7).
The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.