| 别名: | pET31b, pet 31b |
|---|---|
| 质粒类型: | 大肠杆菌表达载体 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5742 bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | C-His |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 备注: | Fuse with ketosteroid isomerase for high yield bioproduction of peptides and small proteins; unique AlwNI cloning site for unidirectional insertion of tandemly repeated peptides. |
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| QC1215 | pET-31b(+) |
5ug质粒 |
¥1000.00 |
The pET-31b(+) vector (Cat. No. 69952-3) is designed for cloning and high-level expression of peptide sequences fused with the 125aa ketosteroid isomerase protein (1). The unique AlwN I cloning site allows the unidirectional insertion of of tandemly repeated peptide coding regions separated by methionine codons. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).