别名: | pET3a |
---|---|
质粒类型: | 大肠杆菌表达载体 |
表达水平: | 高 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4640 bp |
5' 测序引物: | T7 |
5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
载体标签: | N-T7 |
载体抗性: | Ampicillin (氨苄青霉素) |
产品编号 | 产品名称 | 规格 | 价格 |
---|---|---|---|
QC1921 | pET-3a |
5ug质粒 |
¥1000.00 |
The pET-3a-d vectors carry an N-terminal T7•Tag® sequence and BamH I cloning site. These vectors are the precursors to many pET family vectors; the pET-23a-d(+) series corresponds to pET-3a-d but incorporates several additional features. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.