质粒载体

pFastBac1 载体

pFastBac1 载体

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质粒类型: 昆虫细胞表达载体
启动子: Polyhedrin
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4776 bp
5' 测序引物: pFastbac-F: TATTCCGGATTATTCATACC
3' 测序引物: pFastBac-R: ACAAATGTGGTATGGCTGA
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Gentamicin (庆大霉素)
产品编号 产品名称 规格 价格
QC1166 pFastBac1

5ug质粒

¥1000.00
pFastBac1 质粒图谱

The Bac-to-Bac® Vector Kit contains a pFastBac™ 1 vector, as well an expression control vector, intended for use as part of the Bac-to-Bac® Baculovirus Expression System (Cat. No. 10359-016), which enables the efficent production of recombinant baculovirus for expression testing in insect cells. The Bac-to-Bac® System relies on generation of recombinant baculovirus by site-specific transposition in E. coli rather than homologous recombination in insect cells (Figure 1). This system features:

  • Time-saving expression bacmid. With Bac-to-Bac®, the expression cassette of the pFastBac™ vector recombines with the parent bacmid in DH10Bac™ E. coli Competent Cells (not included with this vector kit) to form an expression bacmid. The bacmid is then transfected into insect cells for production of recombinant baculovirus particles.
  • Easy colony screening. The parent bacmid in DH10Bac™ E. coli contains a segment of the lacZα gene. The lacZα gene is disrupted upon transposition of the expression cassette into the bacmid allowing for blue/white selection of recombinants. This makes identification of recombinant colonies easy.

The Bac-to-Bac® Baculovirus Expression System is designed for fast, small-scale production of recombinant baculovirus. The pFastBac™ 1 vector provided by this kit offers the strong polyhedrin promoter for protein expression and a large multiple cloning site for simplified cloning.