质粒类型: | 昆虫细胞表达载体 |
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启动子: | Polyhedrin |
表达水平: | 高 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4857 bp |
5' 测序引物: | pFastbac-F: TATTCCGGATTATTCATACC |
3' 测序引物: | pFastBac-R: ACAAATGTGGTATGGCTGA |
载体标签: | N-His, N-TEV |
载体抗性: | Ampicillin (氨苄青霉素) |
筛选标记: | Gentamicin 庆大霉素 |
产品编号 | 产品名称 | 规格 | 价格 |
---|---|---|---|
QC1169 | pFastBacHT B |
5ug质粒 |
¥1000.00 |
The pFastBac HT vector is supplied with the multiple cloning site in three reading frames (A, B, and C) to facilitate cloning your gene of interest in frame with the N-terminal 6xHis tag. • Strong polyhedrin (PH) promoter for high-level protein expression. • N-terminal 6xHis tag for purification of recombinant fusion proteins using metal-chelating resin and a TEV protease cleavage site for removal of the 6xHis tagfollowing protein purification. • Vector supplied in 3 reading frames for simplified cloning.