One of 9 shuttle vectors (ATCC 87585 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YItype) and yeast marker (LEU2, URA3 or TRP1). This is a YEtype (yeast episomal) vector with the TRP1 marker. The HindIII, PstI and XbaI restriction sites from the TRP1 gene of S. cerevisiae were removed by using oligodirected mutatgenesis.